True confocal scanner leica tcs sp2 user manual

Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. The newest technology is implemented in the leica tcs spe and this system is the only confocal in its class providing true spectral detection. Jun 12, 2012 nanowerk news following on from the outstanding performance of the leica tcs sp5 series, leica microsystems is once again at the forefront of confocal innovation with the new leica tcs sp8. It is recommended setting up a separate user id for each user set up by the system administrator.

The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging. System components overview 1 tcs sp5 scanner 2 main switch board 3 tcs workstation 4 supply unit 5 control panel 6 microscope stand. The leica tcs sp8 combines the fov scanner, for the widest field of view and image format, with a second high speed resonant scanner. The tcs spe is very easy to use and first results are quickly achieved, even by confocal newcomers.

The leica tcs lsi is the first super zoom 3d confocal, offering high resolution plus a large field of view for in vivo imaging. For some cases, avalanche photodiodes have proven to fit best. Manual leica tcs sp ii university of massachusetts. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. Leica tcs sp8 wll confocal smd microscope wellcome. The intensity maximum or center of mass is then assigned the real z. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Theres also the full leica sp8 user manual plus the other leica sp8 confocal manuals. Details the leica tcs sp2 has a system magnification of 3.

Since the lcs software is based on the user administration of windows. Leica tcs sp2 aobs laser scanning spectral confocal microscope. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. Tcs stands for true confocal scanner and sp5 for the 5 spectrophotometer channels this system is equipped with. It thus acquires about 25 images per second at a frame resolution of 512 x 512. You will discover useful features that you breezed over the first time. Leica tcs sp5 ii acoustooptical beam splitter products. Merging both systems into one single device, the tcs sp5 is the ideal solution for imaging core facilities and other multi user environments. Training manual leica tcs sp5 confocal microscope september 2010.

There is the option of the conventional scanner or the fast. The tcs sp2 is the perfect instrument for biomedical research, chemical analysis and material science applications. Leica tcs spe true pointscanning spectral system confocal. Leica tcs sp2 confocal microscope zmbh imaging facility short descripon. True confocal scanner leica tcs sp2 user manual ctac. Synergies confocal tcs sp8 included sted optional mpcars optional smd optional. True confocal scanning tcs is a technique, where only a single, diffraction limited spot is illuminated and observed at a. A true confocal scanner equipped with a true confocal point scanner, the leica tcs spe delivers images of the highest resolution by scanning the specimen in thin optical layers. Leica tcs sp8 has been designed for confocal microscopy with optimal photon efficiency and high speed. If you are interested in these topics or certain applications from the area of optics and confocal microscopy, you. Further help is available via inprogram help within the leica lasaf software.

Online tutorial leica tcs comes with an online tutorial that teaches you how to use the software and hardware. It is recommended setting up a separate user id for each user system administrator. Resonant scanner the resonant scanning system of the leica tcs sp5 works at 8000 hz frequency and enables to record data in both directions of the scan motion. Two of these detectors are very sensitive hyd sp gaaspdetectors. Leica sp5 scanning confocal cdb equipment resource. Leica tcs lsi super zoom confocal microscope medgadget.

Technical specifications and connection requirements for tcs sp2 mp the leica tcs sp2 mp is classified as over. However, the side length of its square pinhole is usually specified in airy disk units, referenced to a standard fixed wavelength of 580 nm, making it independent of the actual overall magnification. Confocal laser scanning microscopy clsm is one of a series of methods to generate slices from microscopic samples by means of optics. This system is based on an inverted leica dmi 6000 cs microscope base with a conventional scanhead equipped with 7 laser lines that provide flexibility for fluorophore excitation and a zgalvo automated stage for zstacks and 3dprojections. Traditionally, photomultiplier tubes have been employed in such systems.

Leica tcs sp2 rs, laser scanning spectral confocal. Synergies confocal tcs sp8 included sted optional mpcars optional falconfcs optional. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained. Leica sp5 confocal microscope umass medical school. Fan on turn laser power level to lowest setting min turn argon laser key to start briefly, and then back to on turn green and red hene lasers on with keys if needed. Make sure that your sample slides are clean and sealed. Leica tcs sp2 aobs laser scanning spectral confocal microscope the leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens.

Mar 07, 2016 first look at a hugely complicated bit of optics kit. Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. Sep 08, 2014 leica microsystems field of view fov scanner offers the largest field number of any confocal point scanning microscope, so that you see more through a wider field of view. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Turn on the ctr control box, fluorescent light for the microscope stand. The laser light emitted from the leica tcs sp2 used in accordance with the instructions is. This leica sp2 platform is equipped with two leica confocal microscopes. Leica microsystems patented threemirror scanner achieves parallaxfree scanning in a large field of view and even illumination. The leica tandem scanner combines two technological solutions in one system. The leica tcs sp5 ii growth confocal system without compromise, the leica tcs sp5 ii confocal system is equipped with the latest technology, offering high sensitivity and superb resolution. Leica tcs sp5 user guide leica sp5sted confocal microscope. The leica tcs sp2 is supplied with the latest version of the licensed leica confocal software. The confocal microscope and accessories the instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy.

Aug 22, 2012 in this article, advantages and disadvantages of different types of sensors for single point true confocal scanning devices are discussed. The new leica tcs sted cw confocal microscope is the most straightforward way to user manual. Leica tcs sp5 user manual national university of singapore. Log into user profile press ctrl, alt and delete simultaneously enter userid and password 5. An introduction from your bosssupervisor is not enough the system has 5 detectors. What should the user of the laser scanning microscope observe. Introduction to confocal laser scanning microscopy leica. Fully motorized leica dm series research microscope upright or inverted true single point, high resolution confocal scanner. Typing your password identifies you as a valid user for this system. Here, a new solution by leica microsystems solves the problem. High speed scanning leica tcs sp8 with tandem scanner.

The leica tcs sp2 true confocal scanner system combines microscopy and video technology with computer electronics, optics and laser technology to offer unprecedented two and threedimensional imaging. Leica tcs sp2 aobs laser scanning spectral confocal. Tcs sp8 smd with white light laser and inverted microscope. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image. The microscope is a leica dmi 6000 cs inverted microscope with a motorized xystage and zdrive motor focus on the microscope stand 100 nm step size as well as a superz galvo stage 1500 m travel range for higher speed and accuracy. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. Download the information on the cell culture dishesor chambers for leica sp8 confocal microscopy here. Leica tcs sp2 laser scanning spectral confocal microscope. Its an online workbook, because you can practically enforce the steps that make up the tutorial to learn how to use the leica tcs software.

The following is the details for confocal platform as well as two microscopes. To learn about the leica confocal software, use the book as a tutorial. For true confocal microscopy, you always want to choose airy 1. The leica confocal microscope system b46 weill hall revised65 startup turn on 3 red buttons on far right. Experienced users can search through the chapters, table of contents, and index to find special topics explained. For sale leica tcs sp2 rs, laser scanning spectral confocal microscope unit is pull from system and i super condition seller assumes all responsibility for this listing. This user manual specifies names of products or services that are trademarks or. User switchable between leica microscopes alignmentfree for lifetime 3 individual ports for external lasers for up to 6 lasers connected simultaneously fibercoupling andor direct coupling of lowpower lasers to confocal unit spectral range of detector optics. Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. Uniting two worlds leica tcs sp5 up to five true spectral confocal channels simultaneously.

The leica tcs sp2 is supplied with the latest version of the licensed leica confocal soft. Combining highperformance optics, the fastest true confocal scanner and the most sensitive detection system available, the leica tcs sp8 is a single. True confocal scanner leica tcs sp2 user manual manualzz. It is dependent though on the numerical aperture of the. This new broadband confocal microscope system perfectly merges two worlds in one. Leica microsystems patented threemirror scanner achieves parallaxfree scanning. Get the best deals on confocal microscope microscopes when you shop the largest online.

To always keep the information up to date, the description of software functions was intentionally omitted from these operating instructions. Specifications of the leica tcs sp5 confocal laser scanning microscope tcs stands for true confocal scanner and sp5 for the 5 spectrophotometer channels this system is equipped with. User guide to the ibif leica tcs sp8 mp confocal microscope. This user manual does not contain any information about basic optical principles or the operating principle of microscopes, confocal systems and the like. Leica sp8 user manuals and brochures download the leica tcs sp8 quick start poster and cores abridged instructions for getting started here. Switch speed in an instant the new leica tcs sp5 ii comes with a tandemscanning system mounted on a sliding device. Leica confocal tcssp2 this is part of the backprojected pinhole calculator. Starting the leica sp5 confocal university of leicester. Leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars imaging native specimens without dyes 32 leica hcs a amplify the power of screening 34 leica tcs sp8 smd learn more from single molecules 36 find the right leica tcs sp8 for your research 38 captures and acknowledgements 39. Leica tcs sp5 confocal molecular imaging center mic. A new development uniting vacuum and silicon technology has led to chimeric sensors, called hybrid detectors hyd.

Leica confocal tcs sp2 this is part of the backprojected pinhole calculator. Leica tcs sp5 confocal laser scanning microscope user guide. Turn on the scanner power 1 on the front of the compact supply unit csu 3. The book usually introduces a set of steps to accomplish a particular task with a distinctive heading. The zmb owns an inverted leica tcs, a tsc sp2 confocal system with upright and inverted. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. Use windex and cotton balls or kimwipes to clean coverslips. All rights to this document are held by leica microsystems cms gmbh. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. This setup enables you to easily switch between conventional and resonant scanner.

Leica tcs sp8 confocal microscope flow cytometrycell. The system is easy to use, and first results are quickly achieved, even by confocal novices. True confocal scanner leica tcs sp2 mp user manual. The microscope is a leica dmi 6000 cs inverted microscope with a motorized xystage and zdrive motor focus on the microscope stand 100 nm step size as well as a. The leica tcs spe confocal is a true pointscanning, spectral system at an affordable price for fluorescence imaging of live or fixed cells. This will create individual directories that can be viewed only by the respective user. Ernest mario school of pharmacy, william levine hall, room 002. The leica confocal software allows you to join image data or edited.

The sample stays intact, and the slicing may be repeated many times. Ebook schematic circuit diagram part workshop manuals. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Enter you name, the date, the time, and the account number in the user log book. The leica tcs sp5 system covers a wide range of imaging speeds from spot recording to realtime imaging in a single truly confocal platform. True confocal scanner leica tcs sp2 user manual manualzz printable.